Comet Labs WN591 Manual de usuario descargar pdf (Pagina 3)

Help! There is a comet in my computer! ii

Figures

Figure 1. The principle of fluorescence and absorption and emission spectra of DNA-

bound ethidium bromide................................................................................................... 4

Figure 2. The path of light through a microscope during observation of fluorescence of

ethidium bromide .............................................................................................................. 6

Figure 3. Light emitted from a comet on a slide is detected as an image ....................................... 8

Figure 4. Information on an image is coded as grey values.......................................................... 10

Figure 5. Segmentation of the comet image.................................................................................. 11

Figure 6. Geometric calibration of the image analysis system...................................................... 12

Figure 7. Scaling of a printed image. Printed images should contain a scale bar......................... 13

Figure 8. Grey values for the head, the tail and the background region of the model

comet image ................................................................................................................... 15

Figure 9. The comet profile for a comet recorded from a microscope slide .................................. 18

Figure 10. The comet profile of the model comet image................................................................. 19

Figure 11. The intensity centroids ................................................................................................... 20

Figure 12. The grabbed and the displayed image........................................................................... 22

Figure 13. Types of images with respect to colour.......................................................................... 22

Figure 14. Look-up tables (LUTs).................................................................................................... 25

Figure 15. Effects of image acquisition settings on the information contained in an image

of a comet....................................................................................................................... 26

Figure 16. The histogram of image grey values .............................................................................. 27

Figure 17. Image contrast and brightness....................................................................................... 29

Figure 18. Image saturation............................................................................................................. 31

Figure 19. Viewing image histograms and grey values in Photoshop............................................. 33

Figure 20. Examples of indicators of the pixel grey value............................................................... 34

Figure 21. Effects of uneven illumination of the field of view .......................................................... 35

Figure 22. Detection of image shading in Photoshop...................................................................... 37

Figure 23. Comparison of an 8-bit and a 10-bit camera.................................................................. 39

Figure 24. The effects of binning ..................................................................................................... 41

Figure 25. Different versions of user interface for adjusting the camera settings ........................... 43

Figure 26. Examples of contrast and brightness settings of the displayed image .......................... 44

Figure 27. Measurement of the comet parameters: an overview.................................................... 45

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Comet Labs WN591 Manual de usuario Pagina 3