Comet Labs WN591 Manual de usuario descargar pdf (Pagina 27)

Help! There is a comet in my computer! 24

same illusion of continuous motion as we have when watching movies, which are also

composed of separate images.

When we click to record an image, we say that the image is grabbed or acquired. Unlike

the live image, the grabbed image is saved in more permanent memory of the computer

for later use in image analysis and measurements.

We can read in the instructions for some comet software packages that measurement is

possible on live or imported (previously recorded) images. Measurement of a truly live

image is not possible, because such an image does not exist in digital technology. The

computer can only perform a measurement on a grabbed image, i.e. a saved image file

where all grey values for all pixels are strictly defined numbers. There are, however,

essentially two approaches to measurements. We can have a slide on the microscope, find a

comet, record an image and measure it immediately (live mode). Or, we can record images

of comets, save them in the computer memory, on a hard disk or on a CD, and do the

measurements later. If we have the possibility to do “live” measurements, our camera is

integrated in the software so that the computer can communicate with it, and we can both

record and measure images without exiting the program. Therefore, if we want to do

“live” measurements, we have to make sure when buying the equipment for the comet

assay that the software package supports our camera.

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Comet Labs WN591 Manual de usuario Pagina 27